polyacrylamide gel electrophoresis การใช้

• The specifically bound DNA is eluted from each individual protein-DNA complex and analyzed by polyacrylamide gel electrophoresis.
• The native enzyme was shown to be made up of two subunit monomers via SDS-polyacrylamide gel electrophoresis.
• The first proteomics experiment was conducted with the invention of two-dimensional polyacrylamide gel electrophoresis ( 2D-PAGE ).
• The title of the paper was " The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis ".
• Proteins can, thus, be separated by net charge in a polyacrylamide gel using either 2-D gel polyacrylamide gel electrophoresis.
• These include liquid chromatography mass spectrometry along with sodium dodecyl sulfate polyacrylamide gel electrophoresis, or liquid chromatography mass spectrometry run in multiple dimensions.
• In humans, rats and mice, CD36 consists of 472 amino acids with a predicted molecular weight of approximately 53, 000 SDS polyacrylamide gel electrophoresis.
• SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques to separate proteins according to their denatured ( unfolded ) state.
• By applying SDS-polyacrylamide gel electrophoresis and subsequent immunoblotting, molecular weights of ? and ?-tubulins were determined to be 48 and 45 kDa, respectively.
• In this dimension, the protein is separated by its isoelectric point ( pI ) and the second-dimension is SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ).
• While monitoring the purification of CEL I using polyacrylamide gel electrophoresis, Yang et al . noticed that there were two nuclease bands that stayed together during all the purification steps.
• Polyacrylamide gel electrophoresis ( PAGE ) is used for separating proteins ranging in size from 5 to 2, 000 kDa due to the uniform pore size provided by the polyacrylamide gel.
• Second, proteins can be separated according to their size or molecular weight via size exclusion chromatography or by SDS-PAGE ( sodium dodecyl sulfate-polyacrylamide gel electrophoresis ) analysis.
• "' Xylene cyanol "'can be used as a color marker, or tracking dye, to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.
• In native polyacrylamide gel electrophoresis ( native PAGE ), proteins remain in their native state and are separated in the electric field following their mass and the mass of their complexes respectively.
• Walter Gilbert and Allan Maxam developed a DNA sequencing method which combined chemicals that cut DNA only at specific bases with radioactive labeling and polyacrylamide gel electrophoresis to determine the sequence of long DNA segments.
• Proteins are usually analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS-PAGE ), by native gel electrophoresis, by preparative gel electrophoresis ( QPNC-PAGE ), or by 2-D electrophoresis.
• The native elution, consisting of the new protein and its interacting protein partners as well as CBP, can now be analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS-PAGE ) or be identified by mass spectrometry.
• In cases of human cysticercosis, diagnosis is a sensitive problem and requires biopsy of the infected tissue or sophisticated instruments . " Taenia solium " eggs and proglottids found in feces, ELISA, or polyacrylamide gel electrophoresis diagnose only taeniasis and not cysticercosis.
• Together they produced the " Weber and Osborn " SDS-PAGE paper, which showed that proteins could be dissolved in sodium dodecyl sulfate ( SDS ), reliably separated by polyacrylamide gel electrophoresis ( PAGE ), visualized by Coomassie Brilliant Blue staining, and their molecular weights determined with reasonable accuracy.